Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis
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چکیده
منابع مشابه
Kinetic studies on depurination and detritylation of CPG-bound intermediates during oligonucleotide synthesis.
Fully protected CPG-immobilized monomer, dimer and trimer oligonucleotides were used to study depurination during the chemical synthesis of oligonucleotides. Disappearance of the oligonucleotide during acid exposure time relative to an internal thymidine standard not subject to depurination was monitored by reverse phase HPLC analysis. Depurination half-times obtained for dichloroacetic acid (D...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1996
ISSN: 1362-4962
DOI: 10.1093/nar/24.15.3053